This step-by-step video clearly demonstrates the correct technique for the execution of the manual assay for the presence FVIII inhibitors. Supplementing the Lab Manual written protocol (Section 28), it also explains the theory behind the assay, details the important reagents, discusses the challenges posed by limited resources, and works through the plotting and interpretation of a series of typical diagnostic cases including immediate acting and time-dependent inhibitors. The accompanying searchable transcript links to the individual sections of the video. A blank FVIII inhibitor assay record sheet and log-linear graph paper are also provided for free download.
Click on the player below to start the video, make sure that your computer speakers are turned on to hear the narration. Below the player is a searchable transcript. Enter a keyword in the search field to identify all the points in the script where it is mentioned. Click on the preceding section title to jump to that point in the video. A list of abbreviations can be found at the end of transcript. Click on the icons below to download the supplementary materials: FVIII inhibitor assay record sheet, log-linear graph paper, and Section 28 of the Lab Manual, Clotting Factor Inhibitor Screen Based on APTT.
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